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. 2020 Sep 9;11(9):728. doi: 10.1038/s41419-020-02926-8

Fig. 4. Prostate cancer-associated transcript 6 (PCAT6) post-transcriptionally regulates VEGFR2 expression.

Fig. 4

a Luciferase reporter assay was performed to examine the luciferase activity of the VEGFR2 promoter by knockdown of PCAT6. b qRT-PCR detected the relative expression of VEGFR2 by PCAT6 depletion or upregulation in triple-negative breast cancer (TNBC) cells. c RNA pull-down assay detected relative enrichment of putative microRNAs (miRNA)s pulled down by biotin-labeled PCAT6. d RIP assay measured the relative expression of miR-4723-5p, PCAT6, and VEGFR2 pulled down by anti-IgG and anti-Ago2. e Binding sequences of PCAT6/VEGFR2 (wild and mutant) and miR-4723-5p. f Luciferase reporter assay was performed to examine the luciferase activity of wild/mutant PCAT6 and VEGFR2 by miR-4723-5p overexpression. g qRT-PCR and western blot examined VEGFR2 expression in different groups. h Western blot analysis of the Akt/mTOR axis-related proteins. **P < 0.01. n.s. no significance.