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. 2020 Sep 9;11(9):728. doi: 10.1038/s41419-020-02926-8

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© The Author(s) 2020, corrected publication 2022

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Fig. 5 — a Western blot examined VEGFR2 expression in the sh-NC, sh-PCAT6#1, and sh-PCAT6#1 + MG132 group. b Western blot evaluated VEGFR2 expression in the sh-NC and sh-PCAT6#1 group by treatment of cycloheximide (CHX). c RNA pull-down silver staining and western blot revealed USP14 as the protein-binding PCAT6. d RIP assay revealed enrichment of PCAT6 pulled down by anti-IgG and anti-USP14. e Co-IP revealed expression of VEGFR2 pulled down by USP14 using USP14 as IP and VEGFR2 as IB. f RIP assay revealed enrichment of VEGFR2 pulled down by USP14 when PCAT6 was knocked down. g Ubiquitination assay of VEGFR2 when PCAT6 was silenced. h Western blotting analysis of VEGFR2 expression in sh-NC and sh-USP14#1 group by treatment of CHX. i qRT-PCR and western blot examined VEGFR2 expression by USP14 knockdown. **P < 0.01. n.s. no significance.