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. 2020 Sep 9;10:14838. doi: 10.1038/s41598-020-71653-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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T7SS mutants display increased PI staining when treated with LA. Live/Dead staining of S. aureus USA300 WT, ΔessC or ΔesxC mutants after growth to OD₆₀₀ of 1.0, without (A) or with treatment with 80 µM (B) linoleic acid. Images are representative of 3 independent experiments. (C) The ratio of SYTO 9: PI fluorescence (live:dead cells) of 25 different fields per strain was quantitated with ImageJ. Means ± SD are shown, n = 3; ***Indicates P < 0.001, **Indicates P < 0.01 using a one-way ANOVA with Tukey’s multiple-comparison test.