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. 2020 Sep 9;10:14838. doi: 10.1038/s41598-020-71653-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The esxC mutant is less able to incorporate LA into its phospholipids. Representative HPLC chromatograms of native phosphatidylglycerol (PG) species of S. aureus USA300 JE2 WT (A) or ΔesxC (B) grown in TSB (top panel) or in TSB supplemented with 10 µM LA (bottom panel), in negative ionisation mode. Relative quantification of the indicated PG species containing an unsaturated FA in LA-treated WT, ΔessC and ΔesxC. The C18:2-containing PG species, C33:2 (C) and total unsaturated fatty acid (C18:2, C20:2 and C22:2) containing exogenous PG species (C33:2, C35:2 and C37:2) (D) are presented as ratios of total PG species. Mean values are shown; error bars represent SD. n = 3, *indicates P < 0.05 using one-way ANOVA with Dunnett’s test.