Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 9;3:498. doi: 10.1038/s42003-020-01211-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Sensorgrams of domains 1–5 truncated protein (10, 15, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, and 120 nM) flowing over GAA-phosphomonoester (a) or GAA phosphodiester (b) and PPT1 (c) surfaces. Inset graphs show Scatchard plots based on average RU value collected over 10-s time intervals at the end of the association phase for each concentration of domains 1–5 (red bar). The calculated K_Ds (−1/slope) values for two binding events are listed and n = 4 independent experiments (standard error of mean is reported). Results from the accompanying competitive inhibition study are displayed as double reciprocal plots (d–f) in which domains 1–5 protein (at 10, 15, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110 or 120 nM concentration) was preincubated for 2 h with 0, 40, 80, or 100-nM PPT1 (as indicated in d) before being flowed over the three different lysosomal enzyme surfaces as indicated in a–c.