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. 2020 Sep 1;32:106242. doi: 10.1016/j.dib.2020.106242
Subject Neurogenetics
Specific Area Psychiatric epigenetics
Type of data Tables, Raw sequencing data
How data was acquired Genomic DNAs were isolated, digested with MspI, gel-purified, treated with bisulfite and subjected to sequencing by RRBS method to obtain 40X coverage of the individual CpG islands
Data format Raw (files): FASTQ files
Analyzed (files): bedGraph files
Parameters for data collection R1 and Dnmt1tet/tet (Tet/Tet) mouse embryonic stem cell lines, Reduced Representation Bisulfite Sequencing, bedGraph files for data visualization.
Description of data collection Genomic DNAs from the cells above were digested with MspI, ligated to adapters, bisulfite converted and amplified to generate sequencing libraries by RRBS protocol. The libraries were sequenced by Next Generation Sequencing.
Data source location Birla Institute of Technology and Science, Pilani
Hyderabad Campus
Hyderabad, India
Data accessibility Repository name: Gene Expression OmnibusData identification number: GSE152817Direct URL to data: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE152817Raw data links for reviewer access:
Related research article S. Saxena, P.A. Maroju, S. Choudhury, A. Anne, K.N. Mohan, Analysis of transcript levels of a few schizophrenia candidate genes in neurons from a transgenic mouse embryonic stem cell model overexpressing DNMT1, Gene. 757 (2020) 144934. https://doi.org/10.1016/j.gene.2020.144934.