Fig. 4.

MiR-155 attenuates neovascularization in chorioallantoic membrane (CAM) assay. (A) The Gene Set Enrichment Analysis (GSEA) plots depicting angiogenesis and VEGF signaling to be enriched and their corresponding statistical metrics in SNB19-miR155 and control cells. (B) Heatmap depicting the relative expression of genes related to angiogenesis using same cells as in A. Shades of blue represent log2 fold change in gene expression. (C) Bar plots showing quantitative PCR data for the relative expression of angiogenesis markers using same cells as in A. (D) Representative images of chick CAM assay using same cells as in A. Scale bar represents 1 cm. (E) Bar plots representing angiogenic properties of the CAM assay using microscopic images analyzed by Angiogenesis analyzer in Image J. Data represent mean ± SEM. *P ≤ 0.05, **P ≤ 0.001 using two-tailed unpaired Student’s t-test.