Subject Area	Earth and Planetary Sciences
More specific subject area	Oceanography: deep sea research
Method name	Hydrothermal-fluid-trap (“fluid-trap”) on advanced mobile pumping system (MPS), and Mobile-GeoMICROBE (MGM) instrumented sled
Name and reference of original method	Cowen, J.P., Copson, D.A., Jolly, J., Hsieh, C.-C., Lin, H.-T., Glazer, B.T., Wheat, C.G., 2012. Advanced instrument system for real-time and time-series microbial geochemical sampling of the deep (basaltic) crustal biosphere. Deep-Sea Res. PT I 61, 43-56.
Resource availability	Flow sensor with Polyvinylidene fluoride (PVDF) wetted parts:https://www.btflowmeter.com/en/flow-meter-products/flow-meters-lowflow-flowmeters-low-flow-turbine-flow-meter-fuel-flow-meters-diesel-fuel-flow-meter-watermeters-paddlewheel-flow-meter-oil-flow-meter-oilflowmeter-waterflowmeter-water-meters-turbineflowmeter-oilflowmeter/chemical-flowmeter-extrem-low-flow-pvdf-miniature-size-q-0003-8-lpm.htmlTedlar® polyvinyl fluoride (PVF) bags: MiDan Co. 12279 Pipeline Ave.,Chino, CA 91710-2142; Phone: (909) 627-1208,https://www.midanco.com/Mylar bags: Jensen Inert Product, 3773 Northwest 126 Ave, Coral Spring, FL, 33065, http://products.jenseninert.com/category/egories-gas-sampling-bags-tedlar-gas-sampling-bags25-port valve system: McLane Research Laboratories, Inc.: https://mclanelabs.com/JACO Fittings: RYAN HERCO Flow Solutions; Phone: 1-800-848-1141.Three-way ball valve fitting: Harrington Industrial Plastics (Hawaii), 91-361 Kaiholo Street Kapolei, Hawaii 96707, U.S.A, Phone: (808) 690-8220Fitting order information:JACO fitting: Fittings: https://www.rhfs.com/Request for Kynar material 1.Male compressional tube fitting- MALE CONN 1/4"OD X 1/4"MT PVDF (10-4-4-K-PG) https://www.rhfs.com/root/0602.033 2.Female compressional tube connection-FEM CONN 1/4"OD X 1/4"T PVDF(25-4-4-K-PG) https://www.rhfs.com/root/0607.033. 3.Bulkhead union, 1/4"OD X 1/4"OD PVDF (20-4-K-PG) https://www.rhfs.com/root.html?cdskeys=0617.033 4.Bulk head nut- BKHD NUT 1/4" PVDF, Jaco Manufacturing (K-O-4-B):https://www.rhfs.com/root/0634.603 5.PVDF GRIPPER NUT 1/4" (K-PG-4) https://www.rhfs.com/root/0634.503 6.PVDF washer for 1/4" screw https://www.extreme-bolt.com/products-pvdf-washers.html 7.High-Strength PVDF Film https://www.mcmaster.com/pvdf-film 8.JACO Union Connector, PVDF, 1/4" Tube PVDF JACO 15-4-K-PG 9.MALE CONN 1/2"OD X 1/2"MT PVDF (10-8-8-K-PG) https://www.rhfs.com/root/0602.066 10.Female compressional tube connection-FEM CONN 1/2"OD X 1/2"T PVDF(25-8-8-K-PG) https://www.rhfs.com/root/0607.066