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. 2020 Sep 8;11(5):e01354-20. doi: 10.1128/mBio.01354-20

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FIG 1 — Stage-dependent trafficking of RhopH2 and CLAG3 in live parasites (A) Ribbon schematics showing C-terminal addition of mVenus and mCerulean tags to rhoph2 and clag3h using CRISPR-Cas9 editing. (B) Immunoblots of indicated parasite clones probed with anti-GFP antibodies that recognize both tags. Lysates harvested at the ring, trophozoite, and schizont stages are shown. Loading control, anti-Band3. Band intensities are not comparable between ring and mature stages. (C) Confocal fluorescence live-cell images of R2mV at the indicated time points after invasion. “Early” indicates infected cells between 0 and 4 h of invasion, as achieved through imaging of live Percoll-enriched schizonts with added erythrocytes. At each stage, line scan analyses are shown in the right panel, based on superimposed dashed white lines. (D) Fluorescence live-cell images of C3mC at indicated time points and corresponding line scan analyses. Images shown for R2mV and C3mC are representative of more than 100 cells visualized over at least 15 experiments.