CenH3 binding defines centromeres in Verticillium dahliae strain JR2. (a) Schematic overview of the chromosomes of V. dahliae strain JR2 showing the normalized CenH3 ChIP-seq read coverage (RPGC normalization in 1-kb bins with 3-kb smoothening), CenH3 enriched regions, GC content, gene density (red line), and repeat density (blue line). (b) Magnification of a 400-kb region containing the centromere is shown for each of the eight chromosomes of V. dahliae strain JR2 (Cen1 to -8) depicting the CenH3 ChIP-seq read coverage (RPGC normalization in 10-bp bins with a 30-bp smoothening) and enrichment, as well as the presence of genes (red) and repetitive elements (blue). Regions carrying the centromere-specific long terminal repeat element VdLTRE9 are highlighted in green.