FIG 2.

ROS output quantification in the nonhost Rm ATCC 11559 and the previously cured host Rm ATCC 52813 cocultivated with and without Mycetohabitans sp. B13 endobacteria isolated from the host ATCC 52813. (A) The nonhost interacting with Mycetohabitans. (B) The nonhost grown without Mycetohabitans. (C) The host interacting with Mycetohabitans. (D) The host grown without Mycetohabitans. In panels A to D, culture plates were stained with yellow, water-soluble NBT, which is reduced by superoxide radicals to blue, water-insoluble formazan, indicating ROS accumulation; white arrowheads show sites of Mycetohabitans or mock inoculation; black arrowheads point to sites of Rm inoculation. (E) Mean RGB color intensity ratios between portions of fungal colonies proximal and distal to the site of Mycetohabitans or mock inoculation. Means are the values from 9 or 10 replicate culture plates. Error bars indicate 1 standard error of the mean. P values from Student's t test are shown next to comparisons.