TABLE 1.
Strains used in this study
| Strain | Genotype | Reference(s) and/or description |
|---|---|---|
| SL1344 | rpsL hisG46 manXE95V menCL148L | 85, 101 |
| SL1344 fis::kan | fis::kan | Kanamycin resistance cassette inserted 63 bp downstream of fis ORF |
| SL1344 dps::cat | Pdps::cat | Chloramphenicol resistance cassette inserted 149 bp upstream of dps ORF |
| SL1344 2xfG | ΔPdpsdps::PfisdusBfis |
dps ORF and upstream/downstream regulatory regions replaced with dusB-fis operon and regulatory regions |
| SL1344 2xdG | ΔPfisdusBfis::Pdpsdps |
dusB and fis ORFs and upstream/downstream regulatory regions replaced with dps ORF and regulatory regions |
| SL1344 2xfO | Δdps::fis | dps ORF replaced with fis ORF |
| SL1344 2xdO | Δfis::dps | fis ORF replaced with dps |
| SL1344 Δfis | Δfis::cat | 19 |
| SL1344 Δdps | Δdps | dps ORF deletion |
| SL1344 GX | ΔPdpsdps::PfisdusBfis ΔPfisdusBfis::Pdpsdps | Exchange of dusB-fis and dps and their cis regulatory regions |
| SL1344 OX | Δdps::fis Δfis::dps | Exchange of fis and dps ORFs |
| SL1344 fis::8xmyc | fis::8xmyc | Deletion of the fis stop codon and insertion of 8 copies of the Myc epitope tag separated by a 21-bp linker |
| SL1344 GX fis::8xmyc | GX fis::8xmyc | Deletion of the fis stop codon and insertion of 8 copies of the Myc epitope tag separated by a 21-bp linker |
| SL1344 OX fis::8xmyc | OX fis::8xmyc | Deletion of the fis stop codon and insertion of 8 copies of the Myc epitope tag separated by a 21-bp linker |
| SL1344 PprgH | PprgH::gfp+::cat | GFP+ fused to SPI-1 promoter, PprgH (99) |
| SL1344 GX PprgH | PprgH::gfp+::cat | GFP+ fused to SPI-1 promoter, PprgH |
| SL1344 OX PprgH | PprgH::gfp+::cat | GFP+ fused to SPI-1 promoter, PprgH |
| SL1344 Δfis PprgH | PprgH::gfp+::cat | GFP+ fused to SPI-1 promoter, PprgH |
| SL1344 Δdps PprgH | PprgH::gfp+::cat | GFP+ fused to SPI-1 promoter, PprgH |
| SL1344 PssaG | PssaG::gfp+::cat | GFP+ fused to SPI-2 promoter, PssaG (99) |
| SL1344 GX PssaG | PssaG::gfp+::cat | GFP+ fused to SPI-2 promoter, PssaG |
| SL1344 OX PssaG | PssaG::gfp+::cat | GFP+ fused to SPI-2 promoter, PssaG |
| SL1344 Δfis PssaG | PssaG::gfp+::cat | GFP+ fused to SPI-2 promoter, PssaG |
| SL1344 Δdps PssaG | PssaG::gfp+::cat | GFP+ fused to SPI-2 promoter, PssaG |
| DH5α |
fhuA2 lacΔU169 phoA glnV44 Φ80' lacZΔM15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 |
102 |
| DH5α pBLUESCRIPT- dusB-dps-cat |
pBLUESCRIPTSK(+)::dusB::dps::cat | dusB-dps operon with cat antibiotic resistance gene |