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Humanin prevented high FFA-induced cytotoxicity in HAECs. Cells were treated with high FFA (1 mM) with or without humanin (25, 50 μM) for 48 h. (A) Morphology of HAECs; (B) release of LDH (****, P < 0.0001 vs vehicle control; ##, p < 0.01 vs FFA treatment group; $$$$, P < 0.0001 vs FFA + 25 μM humanin group, n = 4).
