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Humanin prevented FFA-induced inactivation of the AMPK/ACC signaling pathway in HAECs. Cells were treated with high FFA (1 mM) with or without humanin (25, 50 μM) for 2 h. (A) Phosphorylated and total levels of AMPKα; (B) phosphorylated and total levels of ACC (****, P < 0.0001 vs vehicle control; ##, p < 0.01 vs FFA treatment group; $$$$, P < 0.0001 vs FFA + 25 μM humanin group, n = 4–5).
