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. 2020 Aug 26;5(35):22071–22080. doi: 10.1021/acsomega.0c02094

Figure 5.

Figure 5

Analytical data for (a, b) Vanco-FL-800CW 2, (c, d) Ampho-800CW 3, and (e, f) Vanco-700DX 4. (a) Overlay of the PDA spectra of HPLC peaks corresponding to Vanco-FL (red), IRdye800CW-NHS ester (yellow), and Vanco-FL-800CW (green). (b) Mass spectrum of the product peak (Figure S9) recorded on a UPLC-MS device. (c) Overlay of PDA spectra of the HPLC peak corresponding to amphotericin B (blue), IRdye800CW NHS ester (yellow), and Ampho-800CW (green). (d) Mass spectrum of the product peak (Figure S10) recorded on a UPLC-MS device. (e) Overlay of the UV–vis absorption spectra of vancomycin (blue), IRdye700DX-NHS ester (orange), and Vanco-700DX (green). The spectra were obtained of the pure samples in 1% DMSO in water on a UV–vis spectrophotometer. (f) Mass spectrum of the product peak (Figure S11) recorded on a UPLC-MS device.