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. Author manuscript; available in PMC: 2020 Sep 10.
Published in final edited form as: Nat Plants. 2020 Jul 20;6(8):1008–1019. doi: 10.1038/s41477-020-0724-1

Figure 1. AtPep1 affects phosphorylation of IRR in a time- and concentration-dependent manner.

Figure 1.

a, IRR phosphorylation sites affected by AtPep1 treatment. b, p35S:IRR-3xHA plants were treated with 1 μM solutions of AtPep1 for 0, 10, 20 and 30 min, or (c) 4 and 8 h. d, p35S:IRR-3xHA plants were treated with 0, 0.1, 0.5 and 1 μM solutions of AtPep1 for 30 min. IRR protein was subjected to immunoblot analysis with anti-phospho-Ser and anti-HA antibodies. Experiments in b–d were repeated three times independently, with similar results.