Table 4. SFC Gradient Conditions for the Three Columns Chosen for this Studya.
| BEH
100 × 2.1 |
C18
HSS 150 × 3.0 |
C18
HSS 50 × 3.0 |
|||||
|---|---|---|---|---|---|---|---|
| time | B % | time (TAG) | B % | time (PL) | B % | time | B % |
| 0 | 2 | 0 | 2 | 0 | 2 | 0 | 2 |
| 0.75 | 2 | 1.5 | 2 | 5.0 | 2 | 2.5 | 2 |
| 10.75 | 50 | 16.0 | 20 | 20.0 | 50 | 18.70 | 64 |
| 13.0 | 50 | 17.0 | 20 | 26.0 | 50 | 24.00 | 64 |
| 16.0 | 2 | 20.0 | 2 | 32.0 | 2 | 27.50 | 2 |
| 18.0 | 2 | 22.0 | 2 | 35.0 | 2 | 29.50 | 2 |
The 15 cm C18 column was used for both the neutral lipid (TAG) and phospholipid (PL) methods.