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. 2020 Aug 24;5(35):22289–22298. doi: 10.1021/acsomega.0c02631

Table 4. SFC Gradient Conditions for the Three Columns Chosen for this Studya.

BEH 100 × 2.1
C18 HSS 150 × 3.0
C18 HSS 50 × 3.0
time B % time (TAG) B % time (PL) B % time B %
0 2 0 2 0 2 0 2
0.75 2 1.5 2 5.0 2 2.5 2
10.75 50 16.0 20 20.0 50 18.70 64
13.0 50 17.0 20 26.0 50 24.00 64
16.0 2 20.0 2 32.0 2 27.50 2
18.0 2 22.0 2 35.0 2 29.50 2
a

The 15 cm C18 column was used for both the neutral lipid (TAG) and phospholipid (PL) methods.