Table 1. Fluorescence Lifetime Values of Free ANS and ANS Complexed to CCL2 Wild-Type Proteins and Monomeric Proteins from Murine and Human Speciesa.
| ANS | τ1 (ns) | τ2 (ns) | τ3 (ns) | average lifetime (ns) | χ2 |
|---|---|---|---|---|---|
| ANS | 0.25 (100) | 0.25 | 0.99 | ||
| mCCL2-WT | 0.23 (37) | 2.5 (38) | 9.2 (25) | 3.3 | 1.1 |
| hCCL2-WT | 0.23 (38) | 2.6 (37) | 9.3 (25) | 3.3 | 1.2 |
| mCCL2-P8A | 0.25 (46) | 2.3 (26) | 11.5 (28) | 3.9 | 1.1 |
| hCCL2-P8A | 0.25 (45) | 2.2 (27) | 11.2 (28) | 3.8 | 1.3 |
a
The values of the relative amplitude for each lifetime are presented in the parenthesis.