Figure 2.

Rora^fl/flLysM^Cre/+ mice show decreased weight gain and improved metabolic function associated with increased expression of genes associated with thermogenesis. Groups of Rora^fl/fl and Rora^fl/flLysM^Cre/+ mice were fed a high-fat diet (HFD; 60% fat) for 16 weeks and weight monitored weekly, percentage weight gain was calculated from the starting weight of each animal and starting and final weight is quantified for each group (A, n = 11 Rora^fl/fl; n = 19 Rora^fl/flLysM^Cre/+). E-WAT, I-WAT and S-BAT (subcutaneous brown adipose tissue) weight was determined after 16 weeks on HFD (B, n = 7 Rora^fl/fl; n = 6 Rora^fl/flLysM^Cre/+). Glucose and insulin tolerance tests (GTT and ITT, respectively) were performed on mice after 14 and 15 weeks on HFD respectively (C, n = 8 Rora^fl/fl; n = 6 Rora^fl/flLysM^Cre/+). The serum AST/ALT ratio was calculated from AST and ALT activity tests on the serum from Rora^fl/fl and Rora^fl/flLysM^Cre/+ mice after 16 weeks on HFD (D, n = 8 Rora^fl/fl; n = 6 Rora^fl/flLysM^Cre/+). E-WAT, I-WAT, and S-BAT were excised after 16 weeks on HFD (n = 6 Rora^fl/fl; n = 6 Rora^fl/flLysM^Cre/+). RNA was isolated and expression of genes associated with the metabolic and thermogenic function of the adipose tissue were assessed (E). All data is representative of mean ± SEM. Student's t test: *P < 0.05, **P < 0.01, ***P < 0.001.