Fig. 5.

Agathisflavone promotes a microglial polarization from a M1 to a M2 profile. Organotypic cerebellar slices from P10-12 mice were maintained for 7 DIV and then treated with LPC for 15–17 h, followed by agathisflavone (FAB) at 5 or 10 μM for a further 2 DIV, or 0.1% DMSO vehicle. (A) Microglial profile analyzed by double immunofluoresecence labelling for the M1 pro-inflammatory marker CD16/32 (red) and M2 anti-inflammatory marker CD206 (green), where co-expression appears yellow; scale bar 50 μm. (B, C) Bar graphs showing the number of CD16/32+, CD206+ and CD206+/CD16/32+ cells (B) and the M1/M2 ratio (C); data are expressed as the mean ± SEM (n = 6), *p < 0.05, **p < 0.01, ****p < 0.0001 (comparing control to treatment groups); ‡‡‡‡p < 0.0001 (comparing LPC-DMSO to LPC+FAB5 and LPC+FAB10); One-way ANOVA followed by Tukey’s post-hoc test. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)