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. 2020 Sep 3;79(5):768–781.e7. doi: 10.1016/j.molcel.2020.07.009

Figure 6.

Figure 6

RNF185-MBRL and TEB4 Recognize Distinct Features on Their Membrane Substrates

(A) Schematic overview of the CYP51A1TM chimeras. The AH and the TMD from the Erg11TM ERAD substrate are indicated in purple.

(B) The levels of CYP51A1TM chimeras were analyzed by flow cytometry (based on GFP fluorescence) in the absence or presence of the p97 inhibitor CB-5083 (4 h; 2.5 μM).

(C) The levels of CYP51A1TM and the chimera CYP51A1TMErg11TMD were analyzed by flow cytometry (based on GFP fluorescence) in cells depleted for the indicated genes.

(D) Degradation of CYP51A1TMErg11TMD analyzed upon inhibition of protein synthesis with CHX in cells depleted for the indicated genes. Cell extracts were analyzed by SDS-PAGE and immunoblotting. The graph shows the average of three experiments; error bars represent the standard deviation.

See also Figure S6.