Figure 4.

Growth and phenotype of S and R cells treated by combined BRAF and MEK inhibitors. Cells were cultured in medium containing DMSO (5 µM) or vemurafenib (5 µM) combined with growing concentrations of cobimetinib (5 nM–2.5 µM) for 72 hours. Cell growth was assessed by the dynamic measure of cell index (A). Expression of ligands involved in NK cell/target interactions was determined in cells treated for 48 hours in medium containing DMSO (5 µM) or vemurafenib (5 µM)+cobimetinib (0.5 µM): box plots summarized four experiments (B). CEACAM, carcinoembryonic antigen cell adhesion molecule; DMSO, dimethyl sulfoxide; HLA, human leukocyte antigen; MEK, mitogen-activated protein kinase; MICA, major histocompatibility complex (MHC) class I chain-related protein A; PD-L, programmed cell death ligand; TRAIL-RII, tumor necrosis factor-related apoptosis-inducing ligand receptor II; ULBP, UL16-binding protein.