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. 2009 Sep;90(Pt 9):2209–2220. doi: 10.1099/vir.0.012203-0

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Fig. 5. — HFFF-2 cells do not respond to CpG oligodeoxynucleotides and the response to pI : C is dependent on endosome maturation. (a) Co-localization of CpG–FITC added extracellularly with EEA1 (i, iii; 30 min) and Rab7 (ii, iv; 2 h) with or without NH₄Cl treatment. (b) IRF3 localization in HFFF-2 cells following treatment with pI : C for 6 h, with or without NH₄Cl. nt, Not treated with pI : C. (c) ISG54 mRNA levels following treatment with pI : C for 18 h were quantified by qRT-PCR, normalized to HPRT and represented as percentages relative to pI : C (−) NH₄Cl.