Figure 4.

Hesperetin and cisplatin promote the apoptosis of GC cells via PTEN-PI3K/AKT and mitochondrial pathways. (A) HGC-27 (SGC-7901) cells were incubated with hesperetin (0, 100, 200, and 300 μM) for 24 h, and western blotting was performed to detect the levels of PTEN, AKT, p-AKT, and CytC. *P < 0.05 vs. the control group. (B) HGC-27 (SGC-7901) cells were incubated with the control, 4 μg/ml cisplatin, 200 μM hesperetin, or 4 μg/ml cisplatin + 200 μM hesperetin, and the levels of related proteins were measured using western blotting. *P < 0.05 vs. the control group. All the above data are the mean ± SD from an average of three experiments.