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Energy transfer quenching assay using QD-RBD and AuNP-ACE2. (a) Changes in QD514-RBD photoluminescence (PL) with different ratios of AuNP-ACE2/QD514-RBD; from 0 (noted as “QD”) to 10 (noted as “QD + 10Au”). (b) Energy transfer efficiency as a function of the ratio of AuNP-ACE2 per QD514-RBD; the experimental plots and three different theoretical models. (c) TEM images of clustered AuNPs due to the binding between AuNP-ACE2 and QD514-RBD (ratio = 8).
