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Mobile phase composition and gradient program for HPLC.
| Mobile phase composition | |||
| Solvent A: Water + 2 mM ammonium formate + 0.2% formic acid, pH 2.4 | |||
| Solvent B: Acetonitrile + 2 mM ammonium formate + 0.2% formic acid, pH4.8 | |||
| Time (mins) | %A | %B | Flow rate (μL/min) |
| 0.00 | 90 | 10 | 350 |
| 2.00 | 90 | 10 | 350 |
| 10.00 | 10 | 90 | 350 |
| 11.00 | 10 | 90 | 350 |
| 12.00 | 90 | 10 | 350 |
| 14.00 | 90 | 10 | 350 |
