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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Bone. 2020 Mar 26;136:115329. doi: 10.1016/j.bone.2020.115329

Figure 3. Additional Reduction of Pyrophosphate Does Not Increase Cementogenesis.

Figure 3.

At 26dpn, 2D reconstructions (A) of mouse mandibles exhibit comparable crown morphology, root structures, and alveolar bone (AB) between WT, Ank KO, Enpp1 mutant, and dKO mice. Compared to WT at 60dpn (B, C), cementum is increased in Ank KO, Enpp1asj/asj, and dKO mice (cementum colored in B, C). Cementicles (B, C, white arrows) are evident in Ank KO, Enpp1asj/asj, and dKO mandibles with more prominent cementicles in Enpp1asj/asj and dKO vs. Ank KO mice. Cementum was subdivided into acellular cementum (AC) (lighter color, coronal 2/3 of root in panel C) and cellular cementum (CC) (darker color, apical 1/3 of root in panel C). (D) Quantitative analysis at 26 and 60 dpn shows differences in Ank KO, Enpp1asj/asj, and dKO vs. WT in acellular cementum volume and cellular cementum volume. The dKO mouse does not develop further increased acellular or cellular cementum compared to individual KO mice. De: Dentin; E: Enamel