Expanded acellular cementum (AC) of Ank KO, Enpp1asj/asj, and dKO mice feature strong immunolocalization of bone sialoprotein (BSP) and osteopontin (OPN). Ank KO, Enpp1asj/asj, and dKO mice exhibit increased dentin matrix protein 1 (DMP1) compared to WT (C). Cementicles stained prominently for BSP, OPN, and DMP1. De: Dentin; PDL: Periodontal ligament; AB: Alveolar bone.