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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Bone. 2020 Mar 26;136:115329. doi: 10.1016/j.bone.2020.115329

Figure 7. Pharmacologic Modulation of Pyrophosphate Regulates Ongoing Cementogenesis.

Figure 7.

3D reconstructions (A) of mouse mandibles and molars (M1–M3) at 35–37dpn after a high phosphate and low magnesium “acceleration” diet, including WT control, untreated Enpp1asj/asj, and Enpp1asj/asj mice treated with the ENPP1-Fc protein for 3 weeks. In untreated Enpp1asj/asj mice, ankylosis (white arrows) between alveolar bone (AB) and cementum is evident in 2D heat map reconstructions (B) and histological sections (C), but ankylosis is absent in ENPP1-Fc treated Enpp1asj/asj mice. Heat map images correspond to hydroxyapatite mineral densities (mg/cm3) defined by the color bar in B. Both untreated and treated Enpp1asj/asj mice feature increased OPN immunolocalization in the periodontal ligament (PDL). ENPP1-Fc treated Enpp1asj/asj mice exhibit lower acellular and cellular cementum volumes (AC and CC, respectively) compared to vehicle-treated Enpp1asj/asj mice (D). Alveolar bone volume is normalized in ENPP1-Fc-treated vs. untreated Enpp1asj/asj mice PDL. De: Dentin.