Table 1.

EV71-VLPs preparation and process-achieved quality parameters.

	Harvest wet cell (g/L)	Expression level (mg/L)	Wet cell disrupted (g)	Purification yield(mg/g wet cell)	DLS (nm)	HPLC Purity (%)	Residual host DNA	Residual host cell proteins (%)	Specific activity (U/µg)
Batch #1	242	253	300	0.13	35.3	99.6	<10ng/20μg	0.004	1049
Batch #2	213	270	250	0.15	35.9	99.7	<10ng/20μg	0.012	885
Batch #3	240	246	280	0.11	35.7	99.4	<10ng/20μg	0.019	847