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. 2020 Aug 29;10(23):10680–10696. doi: 10.7150/thno.44250

Figure 3.

Figure 3

miR-339-5p suppresses H/R-induced oxidative stress by targeting p66Shc in vitro. (A-E) Caco-2 cells were transfected with ago-339 or ago-NC and then subjected to H/R. (A) p66Shc protein expression (n=3). (B) Cell viability (n=6). (C, D) Representative fluorescence images and fluorescence quantification of DCFH-DA (C)- and MitoSOX (D)-stained cells. Scale bars, 100 µm (C) and 12.5 µm (D). (E) MnSOD, catalase and NOX2 protein expression (n=3). (F-J) Ago-339 and SHC1-TPmiR-339-5p or a negative control were cotransfected into Caco-2 cells subjected to H/R. (F) p66Shc protein expression (n=3). (G) Cell viability (n=6). (H, I) Fluorescence quantification of DCFH-DA (H)- and MitoSOX (I)-stained cells. (J) MnSOD, catalase and NOX2 protein expression (n=3). *P<0.05, **P<0.01. The values represent the mean ± SD.