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. 2020 Aug 29;10(23):10680–10696. doi: 10.7150/thno.44250

Figure 6.

Figure 6

circ-PRKCB regulates p66Shc expression and H/R-induced oxidative stress by targeting miR-339-5p. (A) Caco-2 cells were transfected with si-circ-PRKCB or si-NC. qRT-PCR was conducted to detect circ-PRKCB and PRKCB mRNA expression (n=4). (B-F) Caco-2 cells were transfected with si-circ-PRKCB or si-NC and then subjected to H/R. (B) p66Shc protein expression (n=3). (C) Cell viability (n=6). (D, E) Representative fluorescence images and fluorescence quantification of DCFH-DA (D)- and MitoSOX (E)-stained cells. Scale bars, 100 µm (D) and 12.5 µm (E). (F) MnSOD, catalase and NOX2 protein expression (n=3). (G-K) Caco-2 cells were cotransfected with circ-PRKCB siRNA, ant-339 or ant-NC and then exposed to H/R. (G) p66Shc protein expression (n=3). (H) Cell viability (n=6). (I, J) Fluorescence quantification of DCFH-DA (I)- and MitoSOX (J)-stained cells. (K) MnSOD, catalase and NOX2 protein expression (n=3). *P<0.05, **P<0.01. The values represent the mean ± SD.