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. 2020 Aug 25;10(23):10634–10651. doi: 10.7150/thno.44278

Figure 1.

Figure 1

LINC00261 is downregulated in PC tissue and is associated with survival. (A) Expression profile of LINC00261 in the two GEO datasets (GSE15471 and GSE16515). (B) LINC00261 expression was assessed by qRT-PCR in paired pancreatic cancer (PC) and adjacent nontumor (ANT) tissues. (C) LINC00261 expression was assessed by qRT-PCR in PC and normal pancreas (NP) tissues. (D) Receiver operating characteristic (ROC) curve analysis was performed on paired PC patient tissues based on LINC00261 mRNA expression. (E) ROC curve analysis was performed on PC and NP tissues based on LINC00261 mRNA expression. (F) Kaplan-Meier analysis of overall survival of PC patients based on LINC00261 TCGA data (PC, N=178, the study group was dichotomized using the median expression value of LINC00261, dotted lines indicate the corresponding 95% confidence intervals for the two curves, analyzed in GEPIA). (G) Kaplan-Meier analysis of disease-free survival of PC patients based on LINC00261 TCGA data (PC, N=178, dotted lines indicate the corresponding 95% confidence intervals for the two curves, analyzed in GEPIA). (H) Kaplan-Meier analysis of overall survival of PC patients based on LINC00261 ISH score data (PC, N=205). (I) Representative images of LINC00261-positive staining are shown in the three independent PC and NP cohorts. (J) LINC00261 expression was assessed by ISH in PC tissue microarrays combining PC samples from three clinical centers and NP samples (Dots with the same ISH score may be overlapped in the figure). (K) Nomogram based on age, clinical stage, differentiation grade and LINC00261 expression level. (L) Validation of the discrimination ability of the nomogram using the bootstrap corrected area under curve (AUC) and 95% confidence interval (CI). (M) Validation of the calibration of the nomogram using the Hosmer-Lemeshow test. (N) Decision curve analysis comparing the standardized net benefit of the following three models: model including tumor stage only; nomogram (as showed in Figure 1K) constructed in the present study but not including LINC00261; nomogram constructed in the present study. (*P < 0.05, **P < 0.01, ***P < 0.001).