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. 2020 Aug 25;10(23):10634–10651. doi: 10.7150/thno.44278

Figure 2.

Figure 2

LINC00261 significantly inhibits PC cell proliferation, migration and invasion in vitro. (A, B) EdU assays were used to assess the cell proliferation ability. Histogram showing the proliferation rates of transfected cells in the corresponding groups. (C, E) Flow cytometric analysis showed that overexpression of LINC00261 significantly increased the proportion of CFPAC-1 cells in the G0/G1 phase and decreased that of cells in the S phase. (D, F) Flow cytometric analysis showed that knockdown of LINC00261 significantly decreased the proportion of PANC-1 cells in the G0/G1 phase and increased that of cells in the S phase. (G, H) CCK8 assays were used to assess the viability of transfected PC cells. (I-K) Transwell and Matrigel assays were used to assess PC cell migration and invasion abilities. Histogram showing the numbers of migrated and invaded PC cells. (L) WB analysis was performed to assess the expression of cell cycle-related markers. (M) WB analysis was performed to assess the expression of EMT-related markers (*P < 0.05, **P < 0.01, ***P < 0.001).