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. 2020 Aug 29;10(23):10652–10664. doi: 10.7150/thno.47933

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In vitro anti-tumor activity. (A) Live cell staining of cells treated with DOX+5FU mixed solutions, the PD5 scaffold, and the intelligent scaffold (IS), respectively. Untreated cells were used as the control. (B) The number of surviving cells after the respective treatments. (C) Drug release profile of the IS under different pH values. (D) The drug uptake in cells after treatment for 10 h. (E) Immunofluorescence staining of treated cells on days 1 and 2. (F) Quantification of DOX fluorescence intensity of cells in panel D. (G) Quantification of Ki67 fluorescence intensity in cells on days 1 and 2 from panel E. (H) Quantification of the Bcl-2/Bax ratio on days 1 and 2 from panel E.