Table 3.

Comparative sensitivity of nitroreductase-expressing HCT116 cells to HCT116 WT cells for various nitro(hetero)aromatic compounds

		R =
Cell Line	PR-104A	EF5	EF3	Pimo	F-miso	RSU1069	HX4
NfsA_Ec	985	1600	1050	71	690	108	52
NfsB_Ec	1643	57	60	2	203	13	103
YcaK_Ec	1	1	1	1	1	1	1
YieF_Ec	2	1	2	1	1	2	1
AzoR_Ec	3	1	1	1	1	2	3
MdaB_Ec	33	1	1	1	1	2	1
WrbA_Ec	4	2	1	1	1	2	1
KefF_Ec	3	1	1	1	1	1	1
YcdI_Ec	1	1	1	1	1	1	1
YdjA_Ec	1	2	2	1	1	1	1
NemA_Ec	14	4	8	2	28	5	4

Cell lines were exposed to compounds for 18 hours at a range of concentrations followed by 5 days growth in drug-free media. IC₅₀ values were determined as the concentration required to inhibit cell growth by 50% of untreated controls. Values in the table represent the fold change in IC₅₀ of the stated cell line relative to HCT116 WT cells. Raw IC₅₀ values are provided in Supplementary table 1.