(A) Choices of workers and queens in regard to soil from favored or non-favored nesting areas in olfactometer bioassays (±SE, n = 5 biological replicates). Control: empty arm. (B) Typical gas chromatographic traces obtained from the soil of favored and non-favored nesting areas. (C) Content of geosmin and 2-methylisoborneol (±SE, n = 6 biological replicates, independent sample t test). (D) GC−EAD profile of geosmin and 2-methylisoborneol on worker and queen antenna. Upper trace: GC−FID. Lower trace: EAG (unit: mV). (E) Attraction of S. invicta to geosmin and 2-methylisoborneol in a Y-tube olfactometer (n ≥ 30 biological replicates, chi-square test). Control: mineral oil. Different letters above bars indicate significant differences according to Kendall’s W test at the 0.05 level. Asterisks indicate significant differences (*P < 0.05, **P < 0.01, *** P < 0.001).