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. Author manuscript; available in PMC: 2022 Feb 21.
Published in final edited form as: Neuroscience. 2020 Feb 28;456:43–49. doi: 10.1016/j.neuroscience.2020.02.020

Figure 1: PSD-95 overexpression blocks NMDA-induced conformational movement in the NMDAR c-terminal domain.

Figure 1:

A) Experimental design.

B) Field of neurons transfected with GluN1-GFP and infected with Sindbis expressing nuclear localization signal tagged (NLS)-mCherry. Scale bar, 20μm

C) (Top) Representative images of neurons expressing GluN1-GFP/GluN1-mCherry and NLS-mCherry or NLS-mCherry + PSD-95 in a solution containing 100μM 7CK, (Bottom) same neurons in the presence of 100μM 7CK and 25μM NMDA. Pseudo-colored pixels indicating fluorescence lifetime of GluN1-GFP (see color scale); scale bar, 5μm.

D) Average FRET efficiency for the indicated conditions; N>17 neurons per condition, individual data points for each neuron are superimposed; *p<0.05, paired t-test.