A) Representative immunofluorescence images of PSD-95 levels in the CA1 region of the hippocampus of mice and rats of indicated ages. Scale bar is 10μm.
B) Quantification of PSD-95 immunofluorescence in animals of indicated ages. N = 2 slices/animal, 3 images/slice, 5 ROIs/image; **p<0.01, ***p<0.001; unpaired t-test.
C) Representative immunoblots of PSD-95 and actin in mice and rat cerebrum. PSD-95 band was normalized by the corresponding actin band and this ratio was then normalized to P35 signal. N=4 (rats) N=6 (mice), individual data points for each experiment are superimposed; *p<0.05; unpaired t-test.