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. 2020 Mar 5;26(6):2254–2262. doi: 10.1038/s41380-020-0698-4

Fig. 1. Hippocampal expression of DR3 and the DR3 ligand TL1A in a rodent model of adolescent intermittent ethanol (AIE) and postmortem human alcoholic hippocampus.

Fig. 1

a Effects of AIE (5.0 g/kg, i.g. 2 days on, 2 days off, postnatal day [P]25–54) on DR3/TNFRSF25+IR or TL1A/TNFSF25+IR expression in the male rat hippocampal dentate gyrus (DG) at P95. AIE exposure increased DR3/TNFRSF25+IR expression (204% of control) compared with control group. AIE exposure also increased TL1A/TNFSF15+IR expression (184% of control). The data were expressed as the numbers of DR3/TNFRSF25+ or TL1A/TNFSF15+ positive cells. Each point is mean ± SEM per mm2 (n = 8/group). b The correlation between DR3+IR and CC3+IR expression in control and AIE groups in the DG of rat brain. c Photomicrographs of confocal images in the hippocampal DG, active caspase-3+ (green) and NeuN+ (red), bar scale = 30 μm. d Increased DR3/TNFRSF25+IR and TL1A/TNFSF15+IR expression in the DG of postmortem AUD human brain. DR3/TNFRSF25+IR (267% of control) and TL1A/TNFSF15+IR (364% of control) expression in the AUD DG were significantly increased compared with control. Data were expressed as the numbers of DR3/TNFRSF25+ or TL1A/TNFSF15+IR positive cells. Each point is mean ± SEM per mm2 (n = 10/group). **p < 0.01 compared with control group by independent samples t-test. e DR3+IR expression in the DG of postmortem human hippocampus (immunohistochemical staining, bar scale = 50 μm).