Figure 5. LSD1 inhibition requires IRF8 for effect on CD24⁺ cDC differentiation.

C-kit⁺ cells were isolated from mouse bone marrow and cultured on MS-5 stroma cells for 14 days in media supplemented with 10 ng/ml Flt3 ligand as described in the “Methods” section. Cultures were treated with PBS vehicle or ORY (0.1 μM) for the 1^st 7 days of the culture period. Measurement of CD24⁺ cDC differentiation was performed using flow cytometry at the end of the culture period. CD24⁺ cDCs were defined by the immunophenotype mouse CD45⁺, lineage-negative (lin^-), MHC-II⁺, CD11c⁺, CD24⁺, CD172^-. (A) Flow cytometry analysis of CD24⁺ cDC populations following culture of wild-type (WT; top) or Irf8^−/− (Irf8-KO; bottom) c-kit⁺ cells treated with PBS vehicle (left panels) or ORY (right panels). (B) Numbers of CD24⁺ cDCs following differentiation of normal WT or Irf8-KO c-kit⁺ cells treated with PBS or ORY. 3–5 technical replicates were performed for each sample and averaged together. Horizontal bars represent the mean value and error bars represent standard deviation. p-values were obtained using unpaired t-test.