Figure 1.

Schematic of experimental setup to investigate ischemic effects of the GluN2D subunit of the NMDA receptor using MALDI-FT-ICR MSI. GluN2D^−/− and wild type mice either did or did not experience ischemic strokes. Both B and C regions of the brain were isolated from each mouse and sectioned using a cryostat. Resulting consecutive tissue sections were then analyzed using MALDI-FT-ICR MSI in both positive (+) and negative (−) ion modes, resulting in 32 tissue sections for analysis. These 32 different sets of imaging data were then annotated using Metaspace.