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. Author manuscript; available in PMC: 2021 Sep 4.
Published before final editing as: Biomaterials. 2020 Mar 4;242:119922. doi: 10.1016/j.biomaterials.2020.119922

Fig. 6.

Fig. 6

ECM deposition and organization within the OM and ES grafts over time. A: H&E staining; B, C: Masson’s trichrome (blue) staining and Sirius red (red) showing the distribution of collagen. D: Immunofluorescence staining (green) showing the distribution of collagen I; E: Verhoeff’s staining (black) showing the presence of elastin. F: Safranin O (pink) staining showing the distribution of GAGs. G: SHG showing elastin and collagen architecture in native aorta and vascular grafts at 12 weeks. H: Statistical analysis of the immunofluorescence area rate of collagen I. The immunofluorescence area of vascular grafts was normalized by that of the native aorta. The dashed line indicates the interface between scaffold and the neointima. Nuclei were counterstained by DAPI (blue). 3D, 2W, 4W and 12W mean 3 days, 2 weeks, 4 weeks and 12 weeks, respectively. Data are represented as the mean ± SEM for each group. *:˖p < 0.05; **: p < 0.01; ***: p < 0.001 (n = 6, two-way-ANOVA followed by Tukey’s post hoc analysis). Scale bar: 100 μm.