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. Author manuscript; available in PMC: 2021 Jan 6.
Published in final edited form as: Nat Neurosci. 2020 Jul 6;23(9):1055–1066. doi: 10.1038/s41593-020-0654-2

Extended Figure 4. Glutamate uncaging evokes Ca2+ transients most reliably in neurons located < 20 μm away from the uncaging site.

Extended Figure 4.

(a) Ratio of Ca2+ transient-experiencing neurons to those without transients binned in 10 μm bins from the uncaging site. (b) Distance of neurons with Ca2+ transients (defined as dF/F>0.5) or no transients from the uncaging point in larvae treated with DMSO or MNI-glutamate, analyzed by Wilcox rank-sum test (n=fish/neurons, n=5/85 DMSO, 9/70 MNI-glutamate).