Table 1.
Predicted mRNA targets of miR-30a-5p, miR-191–5p, miR-195–5p and miR-206–3p.
| Target gene | Gene name | Putative function | Cumulative weighted context++ score | |
|---|---|---|---|---|
| Slc7a10 | solute carrier family 7 (cationic amino acid transporter, y+ system), member 10 | Amino acid transport | −0.57 | |
| rno-miR-30a-5p | Gskip | GSK3B interacting protein | Scaffolding protein | −0.48 |
| Rqcd1 | rcd1 (required for cell differentiation) homolog 1 | mRNA degradation, silencing | −0.43 | |
| Mical1 | microtubule associated monooxygenase, calponin and LIM domain containing 1 | Cytoskeleton structure | −0.42 | |
| Rasd1 | RAS, dexamethasone-induced 1 | Small GTPase | −0.41 | |
| Tmod2 | tropomodulin 2 | Actin binding protein | −0.68 | |
| mmu-miR-191–5p | Zcchc14 | zinc finger, CCHC domain containing 14 | Unknown | −0.51 |
| Neurl4 | neuralized homolog 4 (Drosophila) | Microtubule organizing center formation | −0.48 | |
| Map3k12 | mitogen-activated protein kinase kinase kinase 12 | Protein kinase, non-canonical MAPK signaling | −0.46 | |
| Mapre3 | microtubule-associated protein, RP/EB family, member 3 | Microtubule organization | −0.42 | |
| Mapk8 | mitogen-activated protein kinase 8 | Kinase, proliferation, differentiation, apoptosis | −0.5 | |
| rno- miR-195–5p | Runx1t1 | runt-related transcription factor 1; translocated to, 1 (cyclin D-related) | Transcription factor | −0.43 |
| Traf3 | TNF receptor-associated factor 3 | Adaptor protein, signal transduction | −0.41 | |
| Coro1c | coronin, actin binding protein 1C | Actin binding protein, Rac1 signaling | −0.84 | |
| rno-miR-206–3p | Tpm4 | tropomyosin 4 | Actin binding protein | −0.63 |
| Ptplad1 | protein tyrosine phosphatase-like A domain containing 1 | Fatty acid elongation, Rac1 signaling | −0.59 | |
| Cplx2 | complexin 2 | SNARE machinery | −0.49 | |
| Arhgap21 | Rho GTPase activating protein 21 | GTPase activating protein, cytoskeleton structure | −0.40 | |
The TargetScanMouse database (http://www.targetscan.org/mmu_72/) was searched for genes predicted to be targeted by the miRNA family containing each of the rno-miRNAs elevated in the serum of rats with alcohol consumption – rno-miR-30a-5p, mmu-miR-191–5p, rno-miR-195–5p and rno-miR-206–3p (mouse was used in the case of miR-191–5p as rno-miR-191–5p was unavailable in TargetScan). Gene candidates predicted to be targeted by the specific miRNAs listed were isolated. Brain expression was determined by Allen Brain Atlas (https://mouse.brain-map.org/) or published literature. Brain-relevant predicted targets are ordered by cumulative weighted context++ score, which indicates the predicted repression of the mRNA by a single miRNA from multiple seed sites (Agarwal et al., 2015).