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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: J Mol Cell Cardiol. 2020 Jun 14;145:59–73. doi: 10.1016/j.yjmcc.2020.06.004

Fig. 8.

Fig. 8

The impact of CR-Cyld overexpression on mTORC1 signaling, the expression of Lamp1, Lapm2 and Rab7 and myocardial necrosis in PO-hearts. (A, B) Littermates of male nontransgenic wild type (NG) and CR-Cyld Tg (TG) mice at age of 10 weeks were subject to sham or TAC operations for 2 weeks. LV lysates of these mice were subject to Western blot analysis of (A) CYLD and mTORC1 signaling and (B) the expression of lysosomal membrane proteins of Lamp1 and Lamp2, as well as Rab7. n=4, *, p<0.05 vs. TAC (−) in the same groups, &, p<0.05 vs. NG TAC (−); #, p<0.05 between indicated groups. (C) The impact of Cyld KO on TAC-induced myocardial death. Littermates of wild type (WT) and Cyld KO mice at age of 8 weeks were subject to TAC for 3 days and then received a single intraperitoneal injection of Evans blue dye (EBD) 18 hours prior to heart harvest. Arrows indicate BED positive necrotic myocardial cells. Animal numbers are indicated in the parentheses. (D) A scheme of our working hypothesis. AP, autophagosomes; L, lysosomes; AL, autolysosomes; PO, pressure overload.