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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Cell Calcium. 2020 Jun 13;90:102244. doi: 10.1016/j.ceca.2020.102244

Fig.4.

Fig.4

Ca+ Sparks in hiPSC-CMs recorded with TIRF microscopy. The color traces show the time course of normalized Ca2+-dependent fluorescence changes at the selected color-coded regions (ROI) in a single hiPSC-CM. The images show the example of evolution of sparks at different color-coded regions. The sparks were recorded at 12.3ms/frame, ~80Hz. The teal, green and blue sparks are developed and decayed much faster as compared to the red sparks. The histogram shows the distribution of spark durations of half maximum.