Antibodies |
ERα |
Santa Cruz |
Cat#Sc-8002 |
SRC-3 |
Monoclonal Antibody/Recombinant Protein Expression Core at BCM |
N/A |
AR-Ab1 |
Monoclonal Antibody/Recombinant Protein Expression Core at BCM |
AR Ab clone #705 |
AR-Ab2 |
Monoclonal Antibody/Recombinant Protein Expression Core at BCM |
AR Ab clone #818 |
Flag M2 beads |
Sigma |
Cat#F2426 |
AR |
Santa Cruz |
Cat#Sc-816 |
P300 |
Santa Cruz |
Cat#Sc-584 |
Flag-HRP |
Sigma |
Cat#A8592 |
Chemicals, Peptides, and Recombinant Proteins |
ERα |
Thermo Fisher |
Cat#A15674 |
SRC-3 |
Monoclonal Antibody/Recombinant Protein Expression Core at BCM |
N/A |
P300 |
Monoclonal Antibody/Recombinant Protein Expression Core at BCM |
N/A |
AR |
Monoclonal Antibody/Recombinant Protein Expression Core at BCM |
N/A |
Dynabeads™ M-280 Streptavidin |
Thermo Fisher |
Cat#11205D |
R1881 |
Sigma |
Cat#R0908 |
Glutathione Sepharose 4B |
GE Life Sciences |
Cat#17075601 |
Pierce Fab Micro Preparation Kit |
Thermo Fisher |
Cat#44685 |
Tri-reagent |
Molecular Research Center |
Cat#TR118 |
Turbo DNase |
Thermo Fisher |
Cat#AM2239 |
17β-estradiol |
Sigma |
Cat#E2758 |
Experimental Models: Cell Lines |
HEK 293T/17 |
Tissue Culture Core (BCM) |
Cat#ATCC® CRL-11268™
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Sf9 |
Monoclonal Antibody/recombinant Protein Expression Core (BCM) |
Cat# ATCC® CRL-1711™ |
Bacterial and Virus Strains |
E.coli BL21 (DE3) |
Agilent |
Cat#200131 |
Recombinant DNA |
pCDNA3.1-flag-AR WT/Mut |
This study |
N/A |
pCMV-flag-SRC-3 WT/Mut |
This study |
N/A |
pGEX-SRC-3 fragments |
Yi et al. 2015 Mol. Cell |
N/A |
3XARE-E4 |
This study |
N/A |
Oligonucleotides |
ARE III PCR forward primer |
Thermo Fisher |
N/A |
ARE III PCR reverse primer |
Thermo Fisher |
N/A |
ARE oligonucleotide |
Santa Cruz |
Cat#sc-2551 |
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Deposited Data |
CryoEM maps of AR complexes |
This study |
EMD-22079 and 22080 |
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Software and Algorithms |
EMAN2.2 |
https://cryoem.bcm.edu/cryoem/downloads/view_eman2_versions |
N/A |
Relion3.0 |
https://www3.mrc-lmb.cam.ac.uk/relion/index.php/Download_%26_install |
N/A |
gctf |
https://www2.mrc-lmb.cam.ac.uk/research/locally-developed-software/zhang-software/ |
N/A |
Chimera |
https://www.cgl.ucsf.edu/chimera/download.html |
N/A |
ChimeraX |
https://www.rbvi.ucsf.edu/chimerax/download.html |
N/A |
Other |
SerialEM |
http://bio3d.colorado.edu/SerialEM/ |
N/A |
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