REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
ERα	Santa Cruz	Cat#Sc-8002
SRC-3	Monoclonal Antibody/Recombinant Protein Expression Core at BCM	N/A
AR-Ab1	Monoclonal Antibody/Recombinant Protein Expression Core at BCM	AR Ab clone #705
AR-Ab2	Monoclonal Antibody/Recombinant Protein Expression Core at BCM	AR Ab clone #818
Flag M2 beads	Sigma	Cat#F2426
AR	Santa Cruz	Cat#Sc-816
P300	Santa Cruz	Cat#Sc-584
Flag-HRP	Sigma	Cat#A8592
Chemicals, Peptides, and Recombinant Proteins
ERα	Thermo Fisher	Cat#A15674
SRC-3	Monoclonal Antibody/Recombinant Protein Expression Core at BCM	N/A
P300	Monoclonal Antibody/Recombinant Protein Expression Core at BCM	N/A
AR	Monoclonal Antibody/Recombinant Protein Expression Core at BCM	N/A
Dynabeads™ M-280 Streptavidin	Thermo Fisher	Cat#11205D
R1881	Sigma	Cat#R0908
Glutathione Sepharose 4B	GE Life Sciences	Cat#17075601
Pierce Fab Micro Preparation Kit	Thermo Fisher	Cat#44685
Tri-reagent	Molecular Research Center	Cat#TR118
Turbo DNase	Thermo Fisher	Cat#AM2239
17β-estradiol	Sigma	Cat#E2758
Experimental Models: Cell Lines
HEK 293T/17	Tissue Culture Core (BCM)	Cat#ATCC® CRL-11268™
Sf9	Monoclonal Antibody/recombinant Protein Expression Core (BCM)	Cat# ATCC® CRL-1711™
Bacterial and Virus Strains
E.coli BL21 (DE3)	Agilent	Cat#200131
Recombinant DNA
pCDNA3.1-flag-AR WT/Mut	This study	N/A
pCMV-flag-SRC-3 WT/Mut	This study	N/A
pGEX-SRC-3 fragments	Yi et al. 2015 Mol. Cell	N/A
3XARE-E4	This study	N/A
Oligonucleotides
ARE III PCR forward primer	Thermo Fisher	N/A
ARE III PCR reverse primer	Thermo Fisher	N/A
ARE oligonucleotide	Santa Cruz	Cat#sc-2551
Deposited Data
CryoEM maps of AR complexes	This study	EMD-22079 and 22080
Software and Algorithms
EMAN2.2	https://cryoem.bcm.edu/cryoem/downloads/view_eman2_versions	N/A
Relion3.0	https://www3.mrc-lmb.cam.ac.uk/relion/index.php/Download_%26_install	N/A
gctf	https://www2.mrc-lmb.cam.ac.uk/research/locally-developed-software/zhang-software/	N/A
Chimera	https://www.cgl.ucsf.edu/chimera/download.html	N/A
ChimeraX	https://www.rbvi.ucsf.edu/chimerax/download.html	N/A
Other
SerialEM	http://bio3d.colorado.edu/SerialEM/	N/A