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A) HPP1 ectodomain was detected in the conditioned media of cells transiently transfected with full length HPP1 plasmid. The Δstalk mutant had very little detectable ectodomain in the media, which was similar to control B) Both full length HPP1 plasmid containing HCT116 and stable HPP1 overexpressing transfectants had robust HPP1 ectodomain shed into the media.
