FIGURE 3.

Similar GAG content in cartilage generated out of periosteum in vivo. Cartilage formation was ectopically induced by injecting an agarose biogel (n = 7) with or without the addition of Aggrecan (2% w/v; n = 8) or COMP (0.5 mg/ml; n = 7) under the tibial periosteum of rabbits and after 14 days generated tissue was harvested for analysis. (A) Wet weight was determined for each ectopically generated cartilaginous tissue. (B) GAG content corrected for DNA content (left panel) or for wet weight (right panel) was determined in samples from (A). Each dot represents the determined value for each of these individual generated tissues per group and lines in graphs indicate mean ± SEM. Statistically significant differences (p < 0.05) are shown by an *, ns = not significant.